Dr. Robert Clark's lab carries out basic and translational research relevant to human diseases, with a major focus on mechanisms of the inflammatory response and oxidative stress, as well as the cell biology and biochemistry of human phagocytic cells.
We have contributed to fundamental understanding of the generation of reactive oxygen species (ROS) by the phagocyte NADPH oxidase system, the biological roles of these products, and the genetics and biochemistry of NADPH oxidase deficiency.
In recent years, we have turned our attention to the structure, function, and physiologic roles of several novel non-myeloid members of the NADPH oxidase (NOX) gene family, focusing particularly on the involvement of these oxidases in disease pathogenesis and the transcriptional regulation of genes that are relevant to the biology of aging. Our recent studies include mechanistic work on neurodegenerative disorders, especially Parkinson’s disease.
de Figueiredo ASP, Salmon AB, Bruno F, Jimenez F, Martinez HG, Halade GV, Ahuja SS, Clark RA, DeFronzo RA, Abboud HE, El Jamali A. Nox2 mediates skeletal muscle insulin resistance induced by a high-fat diet. J Biol Chem 290(21):13427-13439, 2015.
Clark RA. Proton pathway paradox: Hv1 H+ channel sustains neutrophil Nox2 activity, yet suppresses HOCl formation. J Leukocyte Biol 99(1):1-4, 2016.
Li G, Chen C, Laing S, Ballard C, Biju KC, Reddick R, Clark RA, Li S. Hematopoietic knockdown of PPARδ reduces atherosclerosis in LDLR-/- mice. Gene Therapy 23(1):78-85, 2016.
Cong L., Muir ER, Chen C, Qian Y, Liu J, Biju KC, Clark RA, Li S, Duong TQ. Multimodal MRI evaluation of the MitoPark mouse model of Parkinson’s disease. PLoS ONE 11(3), e0151884. doi:10.1371, 2016.
Ahuja SK, Manoharan MS, Harper NL, Jimenez F, Hobson, BD, Martinez H, Ingale P, Liu Y-G, Carrillo A, Lou Z, Kellogg DL, Ahuja SS, Rather CG, Esch RE, Ramirez DA, Clark RA, Nadeau K, Andrews CP, Jacobs RL, He W. Preservation of epithelial cell barrier function and muted inflammation in resistance to allergic rhinoconjunctivitis from house dust mite challenge. J Allergy Clin Immunol 139:844-854, 2017.