Dr. Marcel Daadi is an expert in regulated translational research, especially in stem cell therapeutic applications for Parkinson’s disease and brain injuries. He discovered growth and differentiation conditions to direct human neural stem cells toward the dopaminergic lineage. This work produced two hallmark studies, both of scientific importance and therapeutic application for Parkinson’s disease.
In an industrial setting (NeuroSpheres Ltd. and Layton Biosciences Inc.), he developed therapeutic neural stem cell lines for clinical use in stroke, Parkinson’s disease and other diseases and injuries. He is involved in the development of the world’s first cryopreserved neural product manufactured under current Good Manufacturing Practices and transplanted into patients afflicted with stroke at Layton Biosciences Inc., Sunnyvale CA.
At the University of California San Francisco, he was involved in pivotal Investigational New Drug-enabling gene therapy non-human primate studies that allowed Avigen, Inc. to conduct clinical trials for treating patients with Parkinson’s disease. At Stanford University, he started and led the human embryonic stem cell program focused on developing therapeutic stem cell lines for treating ischemic stroke. He also discovered and patented a novel technique of engineering neural cells from pluripotent human embryonic stem cells that is currently in development for treating patients with stroke.
Dr. Daadi's current and future research is centered on the
of technologies to establish pluripotent stem cells, isolate self-renewable
multipotent NSCs and generate specific neuronal lineages, such as dopaminergic
neurons for treating Parkinson’s disease.
reprogramming and genome-editing technologies to model neurological disorders
in vitro and to understand mechanisms mediating disease development and
degenerative processes following injury or disease.
therapeutic stem cell lines in our current Good
Manufacturing Practices (cGMP) facility at the Southwest
National Primate Research Center.
development using nonhuman primates models of a variety of diseases and
applying cell delivery and multimodal molecular imaging techniques to monitor
the safety and efficacy..
engineering of NSCs to investigate the role of optogenetics on their fate after
grafting. These studies will help determine the mechanisms mediating stem cell
graft–host interactions in enhancing neuro-regeneration and restoring function.
Results from our studies are the foundation of translational
research aimed at repairing diseased or injured brain through transplantation
of highly purified NSCs or stimulation of endogenous repair mechanisms.
Barberi T, Shi Q, Lanford RE. (2014) Nonhuman primate models in translational regenerative medicine.Stem Cells Dev. 23, Suppl 1:83-7.
Daadi M.M.,Grueter B.A., MalenkaR.C., Redmond DE, Steinberg G.K.
(2012) Dopaminergic Neurons from Midbrain-Specified Human Embryonic Stem Cell-Derived Neural Stem Cells Engrafted in a Monkey Model of Parkinson's Disease. PLoS ONE, 2012;7(7):e41120.
Daadi M.M., Hu S., Klausner J.Q., Li Z., Sofilos
G., Wu J.C. and Steinberg G.K. (2013) Imaging Neural Stem Cell Graft-Induced Structural Repair in Stroke. Cell Transplantation,
Daadi M.M. (2011) Novel paths towards neural cellular products for neurological disorders. Regenerative Medicine 6(6
Daadi M.M. (2011) Engineering Therapeutic Neural Stem Cell Lines For Parkinson’s Disease. In Embryonic Stem Cells - Recent Advances in Pluripotent Stem Cell-Based Regenerative Medicine. (Craig Atwood, ed.)
InTech Publisher, ISBN 978-953-307-198-5.
Daadi M.M. (2010) The Common path: Tumor suppression in the generation of iPS cells and cancer stem cells. Regenerative
Martinez-Cerdeno V, Noctor SC,
Espinosa A, Ariza J, Parker P, Orasji S, Daadi M.M, Bankiewicz K,
Alvarez-Buylla A, Kriegstein AR (2010) Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats. Cell Stem Cell 6:238-250
Daadi M.M., Davis A., Arac A., Li Z., Maag A.L., Bhatnagar R., Guohua S., Wu J.C. and Steinberg G.K. (2010) Human neural stem cell grafts modify microglial response and enhance axonal sprouting in neonatal hypoxic-ischemic brain injury. Stroke 41:516-523.
Daadi M.M., Li Z., Arac A.,
Grueter B.A., Sofilos M., Malenka R.C., Wu J.C. and
Steinberg G.K. (2009) Molecular and magnetic resonance imaging of human embryonic stem cell-derived neural stem cell grafts in ischemic rat brain. Molecular
Daadi M.M. and Steinberg G.K. (2009) Manufacturing Neurons from human Embryonic Stem Cells Biological and Regulatory Aspects to Develop a Safe Cellular Product for Stroke Cell Therapy. Regenerative Medicine 4:251-263.
Maag A.L. and Steinberg G.K. (2008) Adherent Self-Renewable Human Embryonic Stem Cell-Derived Neural Stem Cell line: Functional Engraftment in Experimental Stroke Model. PLoS ONE 3(2): e1644.