Dr. Ken Hargreaves lab's primary research interests are in the pharmacology of pain and inflammation. A major focus is on pharmacological regulation of unmyelinated "C" fiber nociceptors, as well as their plasticity in response to inflammation or nerve injury. Investigations are in progress evaluating the effects of TRPV1, cannabinoids, opioids, adrenergics, NPY, sex steroids and other drugs on regulating the activity of these fibers.
In addition, we are interested in identifying major classes of inflammatory mediators and associated receptor/signal transduction systems which mediate activation, sensitization and phenotypic plasticity of these primary afferent fibers in response to tissue inflammation. Responses are measured using isolated superfused tissue, primary trigeminal cultures, microdialysis probes implanted in situ, RIA, EIA, real time PCR, electrophysiology, Affymetrex analyses, IHC, ISH, confocal microscopy, behavior, etc.
Boiko N, Medrano G, Montano E, Jiang N, Parrish J, Hargreaves KM, Stockand J, Eaton B. TRPA1 activation in peripheral sensory neurons underlies the ionic basis of pain hypersensitivity in response to vinca alkaloids. s. PLoS ONE 12(10): e0186888. https://doi.org/10.1371/journal.pone.0186888October 30, 2017.
Hargreaves KM, Ruparel S. Role of Oxidized Lipids and TRP Channels in Orofacial Pain and Inflammation. J Dent Res 95:1117-23, 2016. PMID:27307050
Green D, Ruparel S, Gao X, Ruparel N, Patil M, Akopian A, Hargreaves KM. Central activation of TRPV1 and TRPA1 by novel endogenous agonists contributes to mechanical and thermal allodynia after burn injury. Molecular Pain (DOI: 10.1177/1744806916661725) 2016. PMID:27411353
Eskander M, Ruparel S, Green D, Chen P, Por E, Jeske N, Gao X, Flores E, Hargreaves KM. Persistent nociception triggered by nerve growth factor (NGF) is mediated by TRPV1 and oxidative mechanisms. J Neurosci 35:8593-8603, 2015. PMID:26041925