Please note: The University of Texas Health Science Center at San Antonio will now be called "UT Health San Antonio."

Search Program Faculty/Research

Kenneth M. Hargreaves, D.D.S., Ph.D.

Hargreaves photo

RESEARCH

Dr. Ken Hargreaves lab's primary research interests are in the pharmacology of pain and inflammation. A major focus is on pharmacological regulation of unmyelinated "C" fiber nociceptors, as well as their plasticity in response to inflammation or nerve injury. Investigations are in progress evaluating the effects of TRPV1, cannabinoids, opioids, adrenergics, NPY, sex steroids and other drugs on regulating the activity of these fibers. 

In addition, we are interested in identifying major classes of inflammatory mediators and associated receptor/signal transduction systems which mediate activation, sensitization and phenotypic plasticity of these primary afferent fibers in response to tissue inflammation. Responses are measured using isolated superfused tissue, primary trigeminal cultures, microdialysis probes implanted in situ, RIA, EIA, real time PCR, electrophysiology, Affymetrex analyses, IHC, ISH, confocal microscopy, behavior, etc.

Selected Publications

Boiko N, Medrano G, Montano E, Jiang N, Parrish J, Hargreaves KM, Stockand J, Eaton B. TRPA1 activation in peripheral sensory neurons underlies the ionic basis of pain hypersensitivity in response to vinca alkaloids. s. PLoS ONE 12(10): e0186888. https://doi.org/10.1371/journal.pone.0186888October 30, 2017.

Hargreaves KM, Ruparel S. Role of Oxidized Lipids and TRP Channels in Orofacial Pain and Inflammation. J Dent Res 95:1117-23, 2016. PMID:27307050

Green D, Ruparel S, Gao X, Ruparel N, Patil M, Akopian A, Hargreaves KM. Central activation of TRPV1 and TRPA1 by novel endogenous agonists contributes to mechanical and thermal allodynia after burn injury. Molecular Pain (DOI: 10.1177/1744806916661725) 2016. PMID:27411353

Eskander M, Ruparel S, Green D, Chen P, Por E, Jeske N, Gao X, Flores E, Hargreaves KM. Persistent nociception triggered by nerve growth factor (NGF) is mediated by TRPV1 and oxidative mechanisms. J Neurosci 35:8593-8603, 2015. PMID:26041925

Ruparel S, Hargreaves KM, Eskander M, Rowan S, de Almeida J, Roman L, Henry MA. The oxidized linoleic acid metabolite – Cytochrome P450 system (OLAM-CYP) is active in biopsy samples from patients with inflammatory dental pain. Pain 154(11): 2363-71 2013

Green D, Ruparel S, Roman L, Henry M, Hargreaves KMRole of endogenous TRPV1 agonists in a post-burn pain model of partial-thickness injury. Pain 154(11): 2512-20 2013

Loyd D, Henry M, Hargreaves, KM.  Serotonergic Neuromodulation of Peripheral Nociceptors. Sem Cell Develop Biol 24:51-7, 2013

Ruparel S, Green D, Chen P, Hargreaves KMThe cytochrome P450 inhibitor, ketoconazole, inhibits OLAM-mediated peripheral inflammatory pain. Mol Pain 8:73 2012 (doi:10.1186/1744-8069-8-73).

Loyd D, Sun X, Locke E, Salas M, Hargreaves KMSex differences in serotonin enhancement of capsaicin-evoked calcitonin gene-related peptide release from human dental pulp. Pain 153:2061-7, 2012

Ruparel S, Henry M, Akopian A, Patil N, Zeldin D, Roman L, Hargreaves KMPlasticity of cytochrome P450 isozyme expression in rat trigeminal neurons during inflammation. Pain 153:2013-9, 2012

Loyd D, Chen P, Hargreaves KMAntihyperalgesic effects of serotinergics on 5HT and capsaicin-evoked thermal hyperalgesia in the rat. Neuroscience 203:207-15, 2011.

Ruparel S, Hargreaves KM, Eskander M, Rowan S, de Almeida JF, Roman L, Henry MA. Oxidized linoleic acid metabolite-cytochrome P450 system (OLAM-CYP) is active in biopsy samples from patients with inflammatory dental pain. Pain. 2013 Nov;154(11):2363-71.

Green DP, Ruparel S, Roman L, Henry MA, Hargreaves KM. Role of endogenous TRPV1 agonists in a postburn pain model of partial-thickness injury. Pain. 2013 Nov;154(11):2512-20.

Brandfellner HM, Ruparel SB, Gelfond JA, Hargreaves KM. Major blunt trauma evokes selective upregulation of oxidative enzymes in circulating leukocytes. Shock. 2013 Sep;40(3):182-7.

Loyd DR, Henry MA, Hargreaves KM. Serotonergic neuromodulation of peripheral nociceptors. Semin Cell Dev Biol. 2013 Jan;24(1):51-7.

Ruparel S, Henry MA, Akopian A, Patil M, Zeldin DC, Roman L, Hargreaves KM. Plasticity of cytochrome P450 isozyme expression in rat trigeminal ganglia neurons during inflammation. Pain. 2012 Oct;153(10):2031-9.

Ruparel S, Green D, Chen P, Hargreaves KM. The cytochrome P450 inhibitor, ketoconazole, inhibits oxidized linoleic acid metabolite-mediated peripheral inflammatory pain. Mol Pain. 2012 Sep 24;8:73.

Professor & Chair
Endodontics

Education

Ph.D., Physiology, Uniformed Services University of the Health Sciences, 1986

D.D.S., Dentistry, Georgetown University, 1983

B.A., Neurobiology, University of California at Berkeley, 1977

Contact

Email: HARGREAVES@UTHSCSA.EDU


Phone: (210) 567-3381

Research Profile
More Info...

Graduate Students

IMGP/Neuroscience Jacob Boyd (MD, PhD program)

Michael Eskander (DDS, PhD program)