Research in the Stockand laboratory
focuses on ion channels and ion transport. We are particularly interested
in the proteins involved in controlling ion movement into neurons and
epithelial cells. The prior is important to excitability and how central
neurons signal to each other and how peripheral neurons allow us to sense our
environment. Ion channels in the kidney play a central role in
controlling blood pressure as they set circulatory volume and the water and
electrolyte content of plasma. We focus on understanding biologically
important mechanisms that affect physiology. The lab has a long
publication history on the mechanisms of regulation, structure-function
relations and physiologic function of Deg/ENaC ion channels, in which we have
employed electrophysiology, live-cell optical/fluorescence imaging, biochemical
and molecular biological approaches, as well as contemporary molecular genetics
and assessment of renal function, blood pressure and behavior in live animals.
Mironova, E., Bugaj, V., Roos, K.P., Kohan, D.E. andStockand, J.D.
Aldosterone-independent regulation of ENaC by vasopressin in adrenalectomized mice. PNAS. 109:10095-10100, 2012. PMID: 22665796
Stockand, J.D. The role of the epithelial Na+ channel (ENaC) in high AVP but low aldosterone states. Frontiers in Physiology - Renal and Epithelial Physiology. 3:1-5, 2012.
Bugaj, V., Sansom, S.C., Wen, D., Hatcher, L.I., Stockand, J.D. and Mironova, E.Flow-sensitive potassium-coupled ATP secretion modulates ENaC activity in the distal nephron. JBC. 287:38552-38558, 2012. PMID: 23002235
Boiko, N., Kucher, V., Stockand, J.D. and Eaton, B.A. Pickpocket1 is an ionotropic molecular sensory transducer. JBC. 287:39878-39886, 2012. PMID: 23033486
Boiko, N., Kucher, V., Eaton, B.A. and Stockand, J.D. Inhibition of Neuronal Degenerin/epithelial Na+channels by the Multiple Sclerosis Drug 4-Aminopyridine.JBC. 288:9418-9427, 2013. PMID: 23404498
Boiko, N., Kucher V., Wang, B. and Stockand, J.D. Restrictive Expression of Acid-Sensing Ion Channel 5 (Asic5) in Unipolar Brush Cells of the Vestibulocerebellum. Plos One. 9:e91326, 2014. PMID: 24663811
Mironova, E., Chen, Y., Pao, A.C., Roos, K.P., Kohan, D.E., Bugaj, V. and Stockand, J.D. Activation of ENaC by AVP contributes to urinary concentrating mechanism and dilution of plasma. Am. J. Physiol. 308:F237-F243, 2015. PMID: 25391898
Mironova, E., Boiko, N., Bugaj, V., Kucher, V. and Stockand, J.D. Regulation of Na+ excretion and arterial blood pressure by purinergic signaling intrinsic to the distal nephron: consequences and mechanisms. Acta Physiologica. 213:213-221, 2015. PMID: 25154328.
Akitake, B., Ren, Q., Boiko, N., Ni, J., Sokabe, T., Stockand, J.D., Eaton, B.A. and Montell, C. Coordination and Fine Motor Control Depends on Drosophila TRPg. Nature Comm. 6:7288, 2015. PMID: 26028119.
Boiko, N., Medrano, G., Montano, E., Jiang, N., Williams, C.R., Madungwe, N.B., Bopassa, J.C., Kim, C.C.,Parrish, J.Z., Hargreaves, K.M., Stockand, J.D.1 and Benjamin A. Eaton1. 1These authors contributed equally. TrpA1 activation in peripheral sensory neurons underlies the ionic basis of pain hypersensitivity in response to vinca alkaloids. Plos One. 12(10):e0186888, 2017.