Dr. Eugene Sprague's lab is interested in vascular biology with particular emphasis onendothelial biology. Their efforts are currently directed toward exploring the influence of specific flow environments on the endothelial cellular and molecular mechanisms mediating key elements related to atherogenesis and restenosis at sites of stent revascularization.
They are especially interested in the influence of low shear, reversing flow patterns as an important co-initiating factor in early atherogenesis as well as micro-flow disturbances around stent struts that may contribute to stent restenosis. These studies involve examining gene expression and cell surface expression or secretion of these molecules as well as examining the signaling mechanisms involved in transducing the mechanical stimulus of flow into these specific cellular and molecular responses.
Sprague EA. In vivo cardiovascular assays for drug discovery: Evolution of drug-eluting stent. Current Opinion in Investigational Drugs, 2007;8(3):219-225.
Zhang BX, Ma X, Zhang WK, Yeh CK, Lin A, Luo J, Sprague EA, Swerdlow RH, Katz MS. Polyunsaturated fatty acids mobilize intracellular Ca2+ in NT2 human teratocarcinoma cells by causing release of Ca2+ from mitochondria. Am J Physiol Cell Physiol, 2006;290(5):1321-1333.
Sprague EA, Palmaz JC. A model system to assess key vascular responses to biomaterials. J Endovasc Ther. 2005 Oct;12(5):594-604.
Priscilla P. Cherian, Arlene J. Siller-Jackson, Sumin Gu, Xin Wang, Lynda F. Bonewald, Eugene Sprague, and Jean X. Jiang. Mechanical Strain Opens Connexin 43 Hemichannels in Osteocytes: A Novel Mechanism for the Release of Prostaglandin. Mol Biol Cell 16, 3100-3106 , 2005.
Mohan S, Hamuro M, Sorescu GP, Koyoma K, Sprague EA, Jo H, Valente AJ, Prihoda TJ, Natarajan M. IkB-a-dependent regulation of low shear flow induced NF-kB activity: Role of Nitric Oxide. American Journal of Physiology: Cell Physiology 284:C1039-1047, 2003.
Hamuro M, Palmaz JC, Sprague EA, Fuss C, Luo J: Influence of stent edge angle on endothelialization in an in vitro model. J Vasc Interv Radiol 12: 607-611, 2001.
Mohan S, Mohan N, Valente AJ, Sprague EA: Regulation of low shear flow-induced HAEC VCAM-1 expression and monocyte adhesion. Am. J. Physiol. 276 (Cell Physiol. 45): C1100-C1107, 1999.
Sprague EA, Mohan S., Nerem RM. Shear Stress regulation of Monocyte/endothelial cell interactions. J. Vasc. Surg. 29:1138-1140, 1999.
Sprague EA, Luo J, Palmaz JC: Human aortic endothelial cell migration onto stent surfaces under static and flow conditions. JVIR 8:83-92,1997.
Prasad, A.R.S., Logan. S.A., Nerem, R.M., Schwartz, C.J., and Sprague, E.A. Flow related responses of intracellular inositol phosphate levels in cultured aortic endothelial cells.Circ Res 72:827-836, 1993.