Dr. David Dean's research activities focus on three main areas.
1) The first area of interest involves examining how osteoblasts interact with titanium implant surfaces and the role of arachidonic acid metabolites (prostaglandin E2, arachidonic acid, and nonsteroidal antiinflammatory drugs) in regulating osteoblast response. The laboratory has developing methods for measuring changes in gene expression during the first 3-6 hours of culture on an implant surface so that it is possible to examine some of the very earliest cell responses to titanium or other biomaterials.
2) A second area focuses on isolating and characterizing wear debris particles from resin, wear machine fluids, and tissues. The laboratory is actively working on fractionating wear debris particles into micron, sub-micron, and nanometer size ranges. After fractionation, particle preparations will be tested for their effect on osteoblasts; changes in gene expression, as well as differentiation, proliferation, and local factor production, will be assessed.
3) The third area of research is a joint effort with colleagues in Biomedical Engineering at UTSA, Carnegie Mellon University, and Brooke Army Medical Center/Institute for Surgical Research to develop tissue engineering scaffolds for bone repair. The research focuses on developing new materials for regenerating large segments of bone lost due to trauma such as encountered on the battlefield.
Dean DD, Schwartz Z, Liu Y, Blanchard CR, Agrawal CM, Mabrey JD, Sylvia VL, Lohmann CH, and Boyan BD. The Effect of Ultrahigh Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells In Vitro. Journal of Bone and Joint Surgery (American Volume) 81:452-461, 1999.
Dean DD, Schwartz Z, Blanchard CR, Liu Y, Agrawal CM, Lohmann CH, Sylvia VL, and Boyan BD. Ultrahigh Molecular Weight Polyethylene (UHMWPE) Particles Have Direct Effects on Proliferation, Differentiation, and Local Factor Production of MG63 Osteoblast-like Cells. Journal of Orthopaedic Research 17:9-17, 1999.
Lohmann CH, Bonewald LF, Sisk MA, Sylvia VL, Cochran DL, Dean DD, Boyan BD, and Schwartz Z: Maturation State Determines the Response of Osteogenic Cells to Surface Roughness and 1,25-(OH)2D3. Journal of Bone and Mineral Research 15:1169-1180, 2000.
Dean DD, Lohmann CH, Sylvia VL, Köster G, Liu Y, Schwartz Z, and Boyan BD: Effect of Polymer Molecular Weight and Addition of Calcium Stearate on Response of MG63 Osteoblast-like Cells to UHMWPE Particles. Journal of Orthopaedic Research 19:179-186, 2001.
Lohmann CH, Dean DD, Bonewald LF, Schwartz Z, and Boyan BD: Production of Nitric Oxide and Prostaglandin E2 by Osteogenic Cells in Response to Ultra-high Molecular Weight Polyethylene Particles is Dependent on Cell Maturation State. Journal of Bone and Joint Surgery (American Volume) 84:411-419, 2002.
Bannister SR, Lohmann CH, Liu Y, Sylvia VL, Cochran DL, Dean DD, Boyan BD, and Schwartz Z: Shear Force Modulates Osteoblast Response to Surface Roughness. Journal of Biomedical Materials Research 60:167-174, 2002.
Lohmann CH, Dean DD, Köster G, Casasola D, Buchhorn GH, Fink U, Schwartz Z, and Boyan BD: Ceramic and PMMA Particles Differentially Affect Osteoblast Phenotype. Biomaterials 23:1855-1863, 2002.
Lohmann CH, Tandy EM, Sylvia VL, Hell-Vocke AK, Cochran DL, Dean DD, Boyan BD, and Schwartz Z: Response of Normal Female Human Osteoblasts (NHOst) to 17(-Estradiol is Modulated by Implant Surface Morphology. Journal of Biomedical Materials Research 62:204-213, 2002.
Kim HJ, Kim SH, Kim MS, Lee EJ, Oh HG, Oh WM, Park SW, Kim WJ, Lee GJ, Choi NG, Dinh DB, Hardin RR, Johnson K, Sylvia VL, Schmitz JP and Dean DD. Varying Ti-6Al-4V Surface Roughness Induces Different Early Morphologic and Molecular Responses in MG63 Osteoblast-like Cells. Journal of Biomedical Materials Research 74A:366-373, 2005.
Alves CM, Yang Y, Carnes DL, Ong JL, Sylvia VL, Dean DD, Agrawal CM, and Reis RL: Modulating Bone Cells Response onto Starch-based Biomaterials by Surface Plasma Treatment and Protein Adsorption. Biomaterials 28:307-315, 2006.