Dr. Arunabh Bhattacharya's laboratory is interested in the biochemical and molecular mechanisms underlying sarcopenia, the age-related loss of skeletal muscle mass and function that significantly compromises the quality of life and increases the risk of morbidity and mortality in the elderly. Specifically, the laboratory is investigating the role of lipid metabolic pathways that generate oxidized fatty acids (e.g., 5- and 12/15-lipoxygenase) in inflammation and oxidative damage and its impact on sarcopenia.
Recent studies in older humans suggest a strong association between decline in peripheral motor nerve function and decrease in muscle power. Structural changes in peripheral nerves during aging include the loss of axons and demyelination. Myelin sheath produced by Schwann cells that surround the large myelinated nerve fibers is highly enriched in both lipids and proteins, making it highly susceptible to oxidative damage. Using longitudinal measures of peripheral motor nerve and muscle function, the laboratory is investigating if rescuing oxidative damage mediated degenerative changes in peripheral nerves from aging mice protects peripheral nerve dysfunction and the loss of skeletal muscle mass and function.
Finally, pilot studies are underway in the laboratory to investigate the role of mitochondrial oxidative stress and oxidative damage in Traumatic Brain Injury. This project is in collaboration with Dr. Lora Talley Watts, Assistant Professor/Research, Department of Cellular and Structural Biology, UTHSCSA.
(A) Respresentative chromatograms of 5- and 12-HETE in sciatic nerves from young (8 mo) and old (30 mo) C57BL/6 mice that were analyzed by LC-MS/MS.
(B) Quantification of 5-LO, 12/15-LO and COX pathway derived oxidized fatty acids in sciatic nerves from young and old C57BL/6 mice.
Bhattacharya A, Wei R, Hamilton RT, Chaudhuri AR. (2014) Neuronal cells but not muscle cells are resistant to oxidative stress mediated protein misfolding and cell death: Role of molecular chaperones. Biochem Biophys Res Commun. 2014 Apr 18;446(4):1250-4.
Bhattacharya A, Hamilton R, Jernigan A, Zhang Y, Sabia M, Rahman MM, Li Y, Wei R, Chaudhuri A, Van Remmen H. (2014) Genetic ablation of 12/15-lipoxygenase but not 5-lipoxygenase protects against denervation-induced muscle atrophy. Free Radic Biol Med. 2014 Feb;67:30-40.
Wei R, Bhattacharya A, Hamilton RT, Jernigan AL, Chaudhuri AR. (2013) Differential effects of mutant SOD1 on protein structure of skeletal muscle and spinal cord of familial amyotrophic lateral sclerosis: role of chaperone network. Biochem Biophys Res Commun. 2013 Aug 16;438(1):218-23.
Pulliam DA, Bhattacharya A, Van Remmen H. (2013) Mitochondrial dysfunction in aging and longevity: a causal or protective role? Antioxid Redox Signal. 2013 Oct 20;19(12):1373-87.