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Search Program Faculty/Research

Andrew Hayhurst, Ph.D.

Hayhurst photo

RESEARCH

Dr. Andrew Hayhurst's laboratory has a variety of interests mostly related to antibody engineering technology, applications to emerging pathogen countermeasures, tumor targeting and nanotechnology. 

We have recently developed an antibody pipeline that enables selection, screening and assembly of stop-gap assays to native pathogens and toxins within days.  We prefer llama single domain antibodies (sdAb) over other antibody formats owing to their high production levels, modularity and ruggedness (a).  Our method bypasses protein purification for assembling a stop gap assay via the facile step-wise occlusion of biotin (b) and is plug-and-play within any display platform including a novel cell/vector combination built to enable both low and high level sdAb expression for display and production respectively (c).  

Our anti-Filoviral llama sdAb clones appear to outperform our competitors shark sdAb clones showing higher cross-reactivities among the Ebola group (d) and we are rationalizing why the C-terminal domains of Ebolavirus and Marburgvirus nucleoprotein provide such an attractive target for the sdAb formats.

Selected Publications

Sherwood LJ, Hayhurst A. Ebolavirus nucleoprotein C-termini potently attract single domain antibodies enabling monoclonal affinity reagent sandwich assay (MARSA) formulation. PLoS One. 2013;8(4):e61232.

Sherwood LJ, Hayhurst A. Hapten mediated display and pairing of recombinant antibodies accelerates assay assembly for biothreat countermeasures. Sci Rep. 2012;2:807.

Wu M, Park YJ, Pardon E, Turley S, Hayhurst A, Deng J, Steyaert J, Hol WG. Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies. J Struct Biol. 2011 Apr;174(1):124-36.

Conway JO, Sherwood LJ, Collazo MT, Garza JA, Hayhurst A. Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents. PLoS One. 2010 Jan 21;5(1):e8818.

Swain MD, Anderson GP, Zabetakis D, Bernstein RD, Liu JL, Sherwood LJ, Hayhurst A, Goldman ER. Llama-derived single-domain antibodies for the detection of botulinum A neurotoxin. Anal Bioanal Chem. 2010 Sep;398(1):339-48.

Associate Scientist
Virology and Immunology
Texas Biomedical Research Institute

Education

Ph.D.,Virology, National Institute for Medical Research London , England, 1995

Contact

Email: AHAYHURST@TXBIOMED.ORG

Phone: (210) 258-9530

Research Profile
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Graduate Students

Divya Nandamudi
Microbiology & Immunology (Ph.D.)

Tamarand Darling
Microbiology & Immunology (Ph.D.)