Dr. Andrew Hayhurst's laboratory has a variety of interests mostly related to
antibody engineering technology, applications to emerging pathogen
countermeasures, tumor targeting and nanotechnology.
We have recently developed an antibody pipeline that enables
selection, screening and assembly of stop-gap assays to native pathogens and
toxins within days. We prefer llama
single domain antibodies (sdAb) over other antibody formats owing to their high
production levels, modularity and ruggedness (a). Our method bypasses protein purification for
assembling a stop gap assay via the facile
step-wise occlusion of biotin (b) and is plug-and-play within any display
platform including a novel cell/vector combination built to enable both low and
high level sdAb expression for display and production respectively (c).
Our anti-Filoviral llama sdAb clones appear
to outperform our competitors shark sdAb clones showing higher
cross-reactivities among the Ebola group (d) and we are rationalizing why the
C-terminal domains of Ebolavirus and Marburgvirus nucleoprotein provide such
an attractive target for the sdAb formats.
Sherwood LJ, Hayhurst A. Ebolavirus nucleoprotein C-termini potently attract single domain antibodies enabling monoclonal affinity reagent sandwich assay (MARSA) formulation. PLoS One. 2013;8(4):e61232.
Sherwood LJ, Hayhurst A. Hapten mediated display and pairing of recombinant antibodies accelerates assay assembly for biothreat countermeasures. Sci Rep. 2012;2:807.
Wu M, Park YJ, Pardon E, Turley S, Hayhurst A, Deng J, Steyaert J, Hol WG. Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies. J Struct Biol. 2011 Apr;174(1):124-36.
Conway JO, Sherwood LJ, Collazo MT, Garza JA, Hayhurst A. Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents. PLoS One. 2010 Jan 21;5(1):e8818.
Swain MD, Anderson GP, Zabetakis D, Bernstein RD, Liu JL, Sherwood LJ, Hayhurst A, Goldman ER. Llama-derived single-domain antibodies for the detection of botulinum A neurotoxin. Anal Bioanal Chem. 2010 Sep;398(1):339-48.